Composite
Part:BBa_K1500006:Design
Designed by: Kevin Yang Group: iGEM14_UChicago (2014-10-17)
IPTG-GFP MutL
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1060
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1060
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1060
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1060
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870
Design Notes
NA
Source
MutL from BBa_K1500997 was cut out and inserted downstream into the IPTG-GFP backbone plasmid (K1500006).